Definition: A restriction enzyme is a DNA-cutting protein, found mainly in bacteria and archaea, that recognizes a specific short DNA sequence and cleaves the double helix at or near that site. By ...

Substrate specificity is a defining feature of enzyme function, but its molecular underpinnings remain difficult to decode and engineer. Here, we leverage enzyme proximity sequencing (EP-Seq) to ...

Researchers have found a new enzyme, taken from Flavobacterium akiainvivens, that has a much broader specificity than the current leading alternatives. This analysis resulted in the identification of ...

A new artificial intelligence-powered tool can help researchers determine how well an enzyme fits with a desired target, helping them find the best enzyme and substrate combination for applications ...

Herein, we present a method to directly capture substrates of lysine-modifying enzymes via PTM-acceptor residue crosslinking chemistry in living cells, enabling global profiling of substrates of ...